This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Barrett, J. C. Articles by Pollack, R. Search for Related Content PubMed PubMed Citation Articles by Barrett, J. C. Articles by Pollack, R.

Correlation of in Vitro Growth Properties and Tumorigenicity of Syrian Hamster Cell Lines¹

Division of Biophysics, School of Hygiene and Public Health, The Johns Hopkins University, Baltimore, Maryland 21205 [J. C. B., B. D. C., L. O. M., L. M. S., P. O. P. T.], and Department of Biological Sciences, Sherman Fairchild Center for the Life Sciences, Columbia University, New York, New York 10027 [R. P.]

Several in vitro phenotypic characteristics frequently associated with neoplastic cells were examined in a series of spontaneous and benzo(a)pyrene-induced Syrian hamster clonal cell lines which differed in their degree of tumorigenicity. Nonparametric statistical analysis demonstrated cloning efficiency in semisolid agar, enhanced fibrinolytic activity, decreased serum requirement for growth, decreased organization of intracellular actin, and increased cloning efficiency in liquid medium to be correlated with tumorigenicity. These correlations were not only qualitative but also quantitative. This suggests that the factors determining the degree of tumorigenicity of a cell can be cellular growth properties.

¹ This research is supported in part by Contract N01-CP 55713 and Grant P01-CA16043-04 from the National Cancer Institute and on Contract EY-76-S-02-3280 from the Department of Energy.

² Present address: National Institute of Environmental Health Sciences, NIH, P. O. Box 12233, Research Triangle Park, N. C. 27709.

³ Predoctoral Fellow (Training Grant T32-CA09110-04).

⁴ Present address: Department of Biochemical Oncology, Microbiological Associates, Bethesda, Md. 20016.

⁵ To whom requests for reprints should be addressed.

Received 7/24/78. Accepted 1/30/79.

This article has been cited by other articles:

				S. Guha, J. A. Lunn, C. Santiskulvong, and E. Rozengurt Neurotensin Stimulates Protein Kinase C-dependent Mitogenic Signaling in Human Pancreatic Carcinoma Cell Line PANC-1 Cancer Res., May 15, 2003; 63(10): 2379 - 2387. [Abstract] [Full Text] [PDF]

				H. Kameda, J. I. Risinger, B.-B. Han, S. J. Baek, J. C. Barrett, T. Abe, T. Takeuchi, W. C. Glasgow, and T. E. Eling Expression of Gab1 Lacking the Pleckstrin Homology Domain Is Associated with Neoplastic Progression Mol. Cell. Biol., October 15, 2001; 21(20): 6895 - 6905. [Abstract] [Full Text] [PDF]

				S. Jinno, S.-C. Hung, H. Yamamoto, J. Lin, A. Nagata, and H. Okayama Oncogenic stimulation recruits cyclin-dependent kinase in the cell cycle start in rat fibroblast PNAS, November 9, 1999; 96(23): 13197 - 13202. [Abstract] [Full Text] [PDF]

				F. Bost, R. McKay, M. Bost, O. Potapova, N. M. Dean, and D. Mercola The Jun Kinase 2 Isoform Is Preferentially Required for Epidermal Growth Factor-Induced Transformation of Human A549 Lung Carcinoma Cells Mol. Cell. Biol., March 1, 1999; 19(3): 1938 - 1949. [Abstract] [Full Text] [PDF]

				A. Krämer, C.-P. Carstens, and W. E. Fahl A Novel CCAAT-binding Protein Necessary for Adhesion-dependent Cyclin A Transcription at the G(1)/S Boundary Is Sequestered by a Retinoblastoma-like Protein in G(0) J. Biol. Chem., March 22, 1996; 271(12): 6579 - 6582. [Abstract] [Full Text] [PDF]