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Chromosomal DNA Cytophotometry in 20q- Nonspecific Myeloid Disorders¹

Biomedical Sciences Division, Lawrence Livermore Laboratory, Livermore, California 94550 [A. V. C., B. H. M., L. K. A.], and Department of Medicine, University of Chicago and the Franklin McLean Memorial Research Institute,³ Chicago, Illinois 60637 [J. R. T., J. D. R.]

DNA cytophotometry was used to quantify the chromosomal alterations in the bone marrow and blood of three patients with nonspecific myeloid disorders. All patients possessed a population of cells with a morphologically abnormal chromosome 20, del(20)(qll). In two of the patients, the abnormal chromosome 20 showed nearly identical DNA measurements with a net loss of 0.37% of the total autosomal DNA in one patient and 0.38% in the second. The third patient had a net loss of only 0.25% of the autosomal DNA. Analysis of the DNA content of the long arm and short arm of the abnormal No. 20 indicated that all three cases had chromosomal material added to the short arm (0.10 to 0.14% of the autosomal DNA). About 0.50% of the autosomal DNA was deleted from the long arm in two of the patients; only 0.35% of the autosomal DNA was deleted from the long arm in the third case. Within the limit of resolution, there is no evidence that the material lost has been translocated intact to another chromosome. The origin of the 20q- chromosome as the result of an incomplete pericentric inversion is suggested.

¹ This work was performed under the auspices of United States Department of Energy Contract W-7405-ENG-48 and was supported in part by USPHS Grants GM-20291 and CA-16910.

² To whom requests for reprints should be addressed.

³ Operated by the University of Chicago for the United States Department of Energy under Contract EY-76-C-02-0069.

Received 12/11/78. Accepted 4/24/79.