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A Nucleolar Antigen Found in a Broad Range of Human Malignant Tumor Specimens¹

Department of Pharmacology, Baylor College of Medicine, Houston, Texas 77030 [H. B., F. G., R. K. B., F. M. D., K. S.], and Department of Pathology, Veterans Administration Hospital, Houston, Texas 77211 [F. G., P. G.]

With rabbit antibodies to nuclear 0.01 M Tris-HCI, pH 8, extract or "nucleolar preparations" of human HeLa S3 cells and fluorescein-labeled goat anti-rabbit antibodies, bright nucleolar immunofluorescence was observed in 61 of 63 human adenocarcinomas, squamous cell carcinomas, sarcomas, hematological neoplasms, and other malignant tumors. With these antibodies, nucleolar immunofluorescence was not found in 23 normal tissue specimens, 10 benign adenomas and hyperplastic tissues, and 8 specimens of inflammatory diseases. In the nontumorous tissues examined, positive nucleolar fluorescence was found in a few sections of a gastric ulcer and chronic ulcerative colitis which have known propensities for malignant change; these areas may have been undergoing focal malignant changes.

¹ These studies were supported in part by Cancer Research Grant CA-10893, P1, awarded by the National Cancer Institute, Department of Health, Education and Welfare, the Bristol-Myers Fund, and the Pauline Sterne Wolff Memorial Foundation.

Received 1/30/79. Accepted 4/26/79.

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