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Cytotoxic Effects of Chemotherapeutic Drugs on Mouse Testis Cells¹

Section of Experimental Radiotherapy, The University of Texas System Cancer Center, M. D. Anderson Hospital and Tumor Institute, Houston, Texas 77030

Studies of testicular cell killing in mice by several chemotherapeutic drugs have been performed to evaluate the harmful effects of oncolytic agents on reproduction. Seven drugs, Adriamycin, 1-ß-D-arabinofuranosylcytosine, bleomycin, cyclophosphamide, hydroxyurea, vinblastine, and vincristine, given as single injections, were cytotoxic to differentiated spermatogonia. Adriamycin was also highly effective in killing stem cells. The other drugs produced little or no stem cell loss even at doses toxic to the animals. Negligible killing of spermatocytes and spermatids was noted at any dose level. The results demonstrated that oncolytic agents preferentially killed cells at specific stages of the spermatogenic pathway in mice at doses within the clinical range for humans.

¹ This investigation was supported in part by Grants CA-17364 and CA-06294, awarded by the National Cancer Institute, Department of Health, Education, and Welfare. Mice used in this study were maintained in facilities approved by the American Association for Accreditation of Laboratory Animal Care and in accordance with current regulations and standards of the United States Department of Agriculture and NIH, Department of Health, Education, and Welfare.

² Present address: Shell Development Company, One Shell Plaza, P. O. Box 4320, Houston, Texas 77210.

³ To whom requests for reprints should be addressed.

Received 10/11/78. Accepted 6/ 7/79.

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