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Analysis of Transcription during Type C Viral Induction Using Cell Permeabilization Techniques¹

Biological Carcinogenesis Program, Frederick Cancer Research Center, Frederick, Maryland 21701

The reversible cell permeabilization procedure of Castellot et al. has been applied to chemical induction of endogenous type C virus from mouse cells. This procedure has provided a direct demonstration of the -amanitin sensitivity of viral transcription in intact cells at concentrations known to inhibit RNA polymerase II.

¹ This work was supported by Contract NO1-CO-75380 with the National Cancer Institute, NIH, Bethesda, Md. 20205.

² To whom requests for reprints should be addressed.

Received 8/24/79. Accepted 9/20/79.