This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rae-Venter, B. Articles by Reid, L. M. Search for Related Content PubMed PubMed Citation Articles by Rae-Venter, B. Articles by Reid, L. M.

Growth of Human Breast Carcinomas in Nude Mice and Subsequent Establishment in Tissue Culture¹

Department of Biology, University of California, San Diego, La Jolla, California 92093

Thirty-two malignant human breast tumors were implanted s.c. in female nude mice. Seven tumors survived for two passages, and four were established into permanent transplantable tumor lines. The transplantable tumors have retained the histopathology of the original tumor throughout passaging in the nude mice. In addition, two of the transplantable tumors have low concentrations of estrogen receptor.

Tissue culture of the original tumor specimens upon receipt resulted in epithelial outgrowth in 15 of 32 primary cultures. However, no permanent cell lines were established. Attempts to culture 23 tumors frozen with dimethyl sulfoxide upon receipt were unsuccessful. In contrast, establishment of cell strains was successful with tumor specimens cultured following passage in the nude mice; three cell strains were initiated from two of the transplantable tumors.

¹ This work was supported in part by American Cancer Society Grant BC-60-R USPHS Grant CA 11683 to Nathan O. Kaplan, and USPHS Grant CA 1 88885-02 to Gordon Sato.

² Portions of the work reported here were taken from a dissertation submitted to the University of California, San Diego, in partial fulfillment of the requirements for the Ph.D. degree. To whom requests for reprints should be addressed. Present address: Department of Surgery, University of Texas Medical Branch, Galveston, Texas 77550.

³ Supported by NIH Postdoctoral Fellowship Award 5-F-22-AM00274. Present address: Department of Molecular Pharmacology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, N. Y. 10461.

Received 11/27/78. Accepted 11/11/79.

This article has been cited by other articles:

				J. S. Michaelson, E. Halpern, and D. B. Kopans Breast Cancer: Computer Simulation Method for Estimating Optimal Intervals for Screening Radiology, August 1, 1999; 212(2): 551 - 560. [Abstract] [Full Text]