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Chromosome Markers and Evidence for Clone Formation in Lymphocytes of a Patient with Sézary Syndrome¹

Radiation Biology Section, Department of Radiology [J. C. L., M. E. G.] and Department of Internal Medicine [J. H. H.], Southwestern Medical School, The University of Texas Health Science Center at Dallas, Dallas, Texas 75235

Cytogenetic studies of 222 metaphase lymphocytes stimulated by phytohemagglutinin were carried out on a patient diagnosed clinically as having Sézary syndrome. Twenty-two cells (10%) contained 42 to 100 chromosomes. The remaining 200 cells contained 46 chromosomes and revealed evidence of clone formation; 45 were apparently normal diploid cells, but 155 were pseudodiploid with at least one long submetacentric marker in each cell. This marker was shown to have a consistent banding pattern from cell to cell. Of the 25 pseudodiploid cells karyotyped, there were other types of markers present. Normal chromosomes 2 and 17 were missing in all 25 karyotypes. There were seven sets of two cells, each with an identical karyotype, suggesting subclonal formation. Many of the phytohemagglutinin-stimulated nondividing white blood cells had one or more nuclear protrusions. Cytogenetic examination of peripheral lymphocytes may be of value in diagnosing and following the course of this disease.

¹ This work was supported in part by NIH Grant HD06832.

² To whom requests for reprints should be addressed.

Received 6/25/79. Accepted 6/13/80.

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