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Selective Killing of Glucose and Oxygen-deprived HeLa Cells by Hyperthermia¹

Radiation Therapy Research Laboratory, Memorial Sloan-Kettering Cancer Center, New York, New York 10021

We previously have reported that glucose deprivation specifically enhances cell killing by hyperthermia in the absence of oxygen. The present studies were carried out to evaluate the interplay of glucose and oxygen on cell killing by hyperthermia (37 to 42°) for up to 4 hr under varying concentrations of glucose (0 to 1 mg/ml) and oxygen (0 to 21%). pH 7.4 was maintained in all trials. In the absence of oxygen, enhanced cell killing at 40 and 42° was seen with glucose concentrations below 0.1 and 0.25 mg/ml of media, respectively. In the absence of glucose, enhanced killing was seen when oxygen concentrations were below 1 and 2% at 40 and 42°, respectively. The radiosensitivity of the cells was influenced only by the oxygen concentration during irradiation and not by glucose. These results indicate that oxygen and glucose are important modifying factors of cell lethality by hyperthermia.

¹ Supported in part by National Cancer Institute Grants CA-98747, CA-08748, and CA-24791.

² To whom requests for reprints should be addressed.

Received 3/24/80. Accepted 6/26/80.