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Growth of Preneoplastic Mammary Epithelial Cells in Serum-free Medium¹

Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030

The conditions for growing preneoplastic mammary epithelial cells in primary cell cultures with serum-free factor-defined medium were established in a series of experiments. The cells, grown in Dulbecco's modified Eagle's medium with 0.6 mM Ca²⁺, epidermal growth factor, transferrin, insulin, and Na₂SeO₃, were fibroblast free, exhibited saturation densities similar to those of cells grown in serum-containing medium, and actively incorporated [³H]thymidine into DNA, as demonstrated by biochemical and autoradiography assays. The total growth fraction over a seven-day experiment was 77%. Fetuin was sufficient as a serum substitute for attachment of cells to the dish, although it was apparently not necessary for continued growth of the cells. The possible benefits of growing preneoplastic mammary epithelial cells in serum-free factor-defined medium are discussed.

¹ Supported by National Cancer Institute Research Grant CA-11944.

² To whom requests for reprints should be addressed.

Received 3/20/80. Accepted 7/24/80.