This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Newberne, P. M. Articles by Connor, M. Search for Related Content PubMed PubMed Citation Articles by Newberne, P. M. Articles by Connor, M.

Effects of Sequential Exposure to Aflatoxin B₁ and Diethylnitrosamine on Vascular and Stomach Tissue and Additional Target Organs in Rats¹

Department of Nutrition and Food Science, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139

Male weanling rats were given a standard amount (375 µg) of aflatoxin B₁; some were then exposed to dietary diethylnitrosamine (DEN) in varying concentrations ranging from 5 to 80 ppm for 20 weeks. They were maintained for an additional period of time following DEN exposure and then killed for gross and microscopic examination. DEN at 5 ppm did not enhance aflatoxin B₁ liver carcinogenesis. Ten ppm or more of DEN, however, enhanced liver carcinogenesis and introduced two additional target organs; angiosarcomas of the lung and squamous cell carcinomas of the stomach types of tumors which have not been observed after treatment with DEN alone, were noted.

¹ This work was supported by a grant from Hoffmann-LaRoche Inc. (Nutley, N. J.) and by National Institute of Environmental Health Sciences Grant ES00597.

² To whom requests for reprints should be addressed.

Received 1/10/80. Accepted 7/29/80.