This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Rosen, S. W. Articles by Rabson, A. S. Search for Related Content PubMed PubMed Citation Articles by Rosen, S. W. Articles by Rabson, A. S.

Stimulation by N⁶,O^2'-Dibutyryl Cyclic Adenosine 3':5'-Monophosphate of Ectopic Production of the Free ß Subunit of Chorionic Gonadotropin by a Human Brain Tumor Cell Line

Clinical Endocrinology Branch, National Institute of Arthritis, Metabolism, and Digestive Diseases [S. W. R., I. C., B. D. W.], and Laboratory of Pathology, National Cancer Institute [J. S. T., A. S. R.], Bethesda, Maryland 20205

Previous studies have favored a basic difference in the regulation of specialized protein production by cells derived from the usual tissue of origin (eutopic) and cancer cells derived from a tissue not normally producing the protein (ectopic). Thus N⁶,O^2'-dibutyryl cyclic adenosine 3':5'-monophosphate was believed to stimulate only eutopic (but not ectopic) chorionic gonadotropin production, and butyrate to stimulate only ectopic (but not eutopic). However, in CBT, a human brain tumor cell line, we find that N⁶,O^2'-dibutyryl cyclic adenosine 3':5'-monophosphate, but not butyrate, stimulated ectopic production of the ß subunit of chorionic gonadotropin. We conclude that neither butyrate nor cyclic adenosine 3':5'-monophosphate derivatives reliably discriminate ectopic from eutopic regulation.

¹ To whom requests for reprints should be addressed, at Clinical Endocrinology Branch, National Institute of Arthritis, Metabolism, and Digestive Diseases. Building 10, Room 8N315, Bethesda, Md. 20205.

Received 5/ 1/80. Accepted 8/ 5/80.