This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Loewenstein, P. M. Articles by Gerard, G. F. Search for Related Content PubMed PubMed Citation Articles by Loewenstein, P. M. Articles by Gerard, G. F.

Distribution of DNA Polymerase Cm in Normal and Malignant Human Tissues¹

Institute for Molecular Virology, St. Louis University School of Medicine, St. Louis, Missouri 63110

The distribution in human tissues has been determined of the new DNA polymerase activity designated DNA polymerase Cm, first isolated from the human melanoma cell line A-375. Tissue samples were fractionated by ion exchange chromatography on diethylaminoethyl cellulose and phosphocellulose and by affinity chromatography on poly(2'-O-methylcytidylate)-Sepharose. DNA polymerase activity was monitored with poly(2'-O-methylcytidylate)·oligodeoxyguanylate and poly(adenylate)·oligodeoxyribothymidylate, the most specific and most sensitive template primers, respectively, for DNA polymerase Cm. Tissues were scored as to presence or absence of detectable DNA polymerase Cm activity as well as to their validity for scoring dependent on content of total DNA polymerase activity. On this basis, seven of 14 malignant and none of 11 normal or embryonic tissues were found to contain detectable levels of DNA polymerase Cm.

¹ Supported by Contract NO1 CP 61049 within the Virus Cancer Program of the National Cancer Institute.

² To whom requests for reprints should be addressed.

Received 4/18/80. Accepted 8/15/80.