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An in Vitro Assay Procedure to Test Chemotherapeutic Drugs on Cells from Human Solid Tumors¹

Urologic Research Laboratory, Division of Urology, Department of Surgery, Duke University Medical Center, Durham, North Carolina 27710 [S. S., R. A. B., K. R. S., D. F. P.], and Research Service, Veterans Administration Medical Center, Durham, North Carolina 27705 [R. A. B., D. F. P.]

An in vitro assay was developed to measure the chemotherapeutic drug susceptibility of cells from human tumors. The assay utilized live cells, freshly isolated from tumor tissue, which were incubated for a short period in vitro. The druginduced inhibition of incorporation of radiolabeled precursor into DNA, RNA, and protein was measured. The assay is sensitive to concentrations of chemotherapeutic drugs in the therapeutic range and is reproducible when tested with replicates of the same tumor cell population.

¹ Supported in part by Grants CA15972 and CA14930 from the National Cancer Institute through the National Bladder Cancer Project; and by the Medical Research Service of the Veterans Administration.

² To whom requests for reprints should be addressed, at Box 3813, Duke University Medical Center, Durham, N. C. 27710.

³ Present address: 1720 Woodside Drive, Woodland, Calif. 95695.

Received 3/19/79. Accepted 8/20/80.