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Activation of Cyclophosphamide for in Vitro Testing of Cell Sensitivity¹

Urologic Research Laboratory, Division of Urology, Department of Surgery, Duke University Medical Center, Durham, North Carolina 27710 [S. S., K. R. S., D. F. P., R. A. B.], and Research Service, Veterans Administration Hospital, Durham, North Carolina 27705 [D. F. P., R. A. B.]

Two methods are described for the activation of cyclophosphamide by a liver microsome preparation. These procedures were applicable to an assay in vitro which tests the sensitivity of tumor cells to the drug. Satisfactory results were obtained either by pretreatment of the cyclophosphamide and removal of the microsomes before testing or by the somewhat simpler procedure of mixing drug, microsomes, and test cells for the assay. Microsome treatment of bleomycin gave a smaller increase in activity, and much smaller effects were seen on some other drugs.

¹ Supported in part by Grants CA15972 and CA14930 from the National Cancer Institute through the National Bladder Cancer Project and by the Medical Research Service of the Veterans Administration.

² To whom requests for reprints should be addressed, at Box 3813, Duke University Medical Center, Durham, N. C. 27710.

³ Present address: 1729 Woodside Drive, Woodland, Calif. 95695.

Received 2/20/80. Accepted 8/20/80.