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Stimulation of DNA Synthesis in Calcium-deprived T51B Liver Cells by the Tumor Promoters Phenobarbital, Saccharin, and 12-O-Tetradecanoylphorbol-13-acetate¹

Animal and Cell Physiology Section, Division of Biological Sciences, National Research Council of Canada, Ottawa, Canada K1A OR6

Extracellular calcium deprivation arrested the proliferative development of T51B rat liver cells in late G₁ or S phases. These arrested cells initiated DNA synthesis within an hr after addition of calcium or a tumor promoter such as phorbol-12,13-didecanoate, phenobarbital, saccharin, or 12-O-tetradecanoylphorbol-13-acetate. Nonpromoting relatives of 12-O-tetradecanoylphorbol-13-acetate such as phorbol, 4-O-methyl-12-O-tetradecanoylphorbol-13-acetate, and 4-phorbol-12,13-didecanoate were unable significantly to induce the calciumdeprived cells to initiate DNA synthesis. It is suggested that an ability to elicit a prompt DNA-synthetic response from calciumdeprived cells might be a useful in vitro indicator of tumor-promoting potential.

¹ Issued as National Research Council of Canada No. 18736

Received 12/18/79. Accepted 9/15/80.