| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
In Vitro Pathogenesis Section, Laboratory of Experimental Pathology [S. H. Y., B. K.] and Dermatology Branch [J. R. S.], National Cancer Institute; Laboratory of Developmental Biology and Anomalies, National Institute of Dental Research [J. R. S.]; and Microbiological Associates [P. H. N.], Bethesda, Maryland, 20205
Primary mouse epidermal cells underwent spontaneous malignant transformation in culture. Twelve malignant epidermal cell lines were established which produced squamous cell carcinomas in syngeneic hosts. These lines were used to define criteria for recognizing transformed epidermal cells in vitro. Growth in suspension in agar, agarose, or Methocel was minimal for 11 of the lines. All lines tested retained specific epidermal antigens (pemphigus, pemphigoid, keratin) by indirect immunofluorescence, but keratin content was reduced when quantified by radioimmunoassay. Basal activity of ornithine decarboxylase and activity induced by the tumor promoter 12-O-tetradecanoylphorbol-13-acetate were variable among lines. All malignant lines as well as normal epidermal cells grew well at reduced extracellular calcium concentrations. When the extracellular calcium was elevated, normal cells ceased proliferation, terminally differentiated, and sloughed from the culture dish, while malignant cells continued to proliferate although they expressed differentiative functions. These results indicate that malignant transformation in epidermis is associated with a fundamental alteration in the program of terminal differentiation which allows some cells to escape the proliferative block and cell death which accompanies differentiation in normal keratinocytes. This alteration should be useful to select for transformants during the process of carcinogenesis in vitro.
1 Portions of this work were performed under NCI Contract NO1-CP-02199.
2 To whom requests for reprints should be addressed.
3 Intergovernmental Personnel Act (IPA) Visiting Scientist from the Department of Dermatology, New York University Medical Center, New York, N. Y.
Received 6/24/80. Accepted 9/16/80.
This article has been cited by other articles:
|
|
 |
|
  G. Ramos, A. Y. Limon-Flores, and S. E. Ullrich JP-8 Induces Immune Suppression via a Reactive Oxygen Species NF-{kappa}{beta}-Dependent Mechanism Toxicol. Sci., March 1, 2009; 108(1): 100 - 109. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Ota and H. Sasaki Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling Development, December 15, 2008; 135(24): 4059 - 4069. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. T. Bility, M. K. Devlin-Durante, N. Blazanin, A. B. Glick, J. M. Ward, B. H. Kang, M. J. Kennett, F. J. Gonzalez, and J. M. Peters Ligand activation of peroxisome proliferator-activated receptor {beta}/{delta} (PPAR{beta}/{delta}) inhibits chemically induced skin tumorigenesis Carcinogenesis, December 1, 2008; 29(12): 2406 - 2414. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Hwang, T. Mehrani, S. E. Millar, and M. I. Morasso Dlx3 is a crucial regulator of hair follicle differentiation and cycling Development, September 15, 2008; 135(18): 3149 - 3159. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Duverger, D. Lee, M. Q. Hassan, S. X. Chen, F. Jaisser, J. B. Lian, and M. I. Morasso Molecular Consequences of a Frameshifted DLX3 Mutant Leading to Tricho-Dento-Osseous Syndrome J. Biol. Chem., July 18, 2008; 283(29): 20198 - 20208. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Luke, H. Zaim, I. Karakesisoglou, V. M. Jaeger, L. Sellin, W. Lu, M. Schneider, S. Neumann, A. Beijer, M. Munck, et al. Nesprin-2 Giant (NUANCE) maintains nuclear envelope architecture and composition in skin J. Cell Sci., June 1, 2008; 121(11): 1887 - 1898. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Sadagurski, S. Yakar, G. Weingarten, M. Holzenberger, C. J. Rhodes, D. Breitkreutz, D. LeRoith, and E. Wertheimer Insulin-like growth factor 1 receptor signaling regulates skin development and inhibits skin keratinocyte differentiation. Mol. Cell. Biol., April 1, 2006; 26(7): 2675 - 2687. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. F. Fraga, M. Herranz, J. Espada, E. Ballestar, M. F. Paz, S. Ropero, E. Erkek, O. Bozdogan, H. Peinado, A. Niveleau, et al. A Mouse Skin Multistage Carcinogenesis Model Reflects the Aberrant DNA Methylation Patterns of Human Tumors Cancer Res., August 15, 2004; 64(16): 5527 - 5534. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Kogo, T. Aiba, and T. Fujimoto Cell Type-specific Occurrence of Caveolin-1{alpha} and -1{beta} in the Lung Caused by Expression of Distinct mRNAs J. Biol. Chem., June 11, 2004; 279(24): 25574 - 25581. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Duverger, L. Paslaru, and M. Morange HSP25 Is Involved in Two Steps of the Differentiation of PAM212 Keratinocytes J. Biol. Chem., March 12, 2004; 279(11): 10252 - 10260. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Tanaka, H. Nakanishi, S. Kakunaga, N. Okabe, T. Kawakatsu, K. Shimizu, and Y. Takai Role of Nectin in Formation of E-Cadherin-based Adherens Junctions in Keratinocytes: Analysis with the N-Cadherin Dominant Negative Mutant Mol. Biol. Cell, April 1, 2003; 14(4): 1597 - 1609. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Takahashi, H. Kosaka, K. Oritani, W. R. Heath, J. Ishikawa, Y. Okajima, M. Ogawa, S.-i. Kawamoto, M. Yamada, H. Azukizawa, et al. A New IFN-Like Cytokine, Limitin Modulates the Immune Response Without Influencing Thymocyte Development J. Immunol., September 15, 2001; 167(6): 3156 - 3163. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Dong, E. Loukinova, Z. Chen, L. Gangi, T. I. Chanturita, E. T. Liu, and C. Van Waes Molecular Profiling of Transformed and Metastatic Murine Squamous Carcinoma Cells by Differential Display and cDNA Microarray Reveals Altered Expression of Multiple Genes Related to Growth, Apoptosis, Angiogenesis, and the NF-{{kappa}}B Signal Pathway Cancer Res., June 1, 2001; 61(12): 4797 - 4808. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. B. Sunwoo, Z. Chen, G. Dong, N. Yeh, C. C. Bancroft, E. Sausville, J. Adams, P. Elliott, and C. Van Waes Novel Proteasome Inhibitor PS-341 Inhibits Activation of Nuclear Factor-{{kappa}}B, Cell Survival, Tumor Growth, and Angiogenesis in Squamous Cell Carcinoma Clin. Cancer Res., May 1, 2001; 7(5): 1419 - 1428. [Abstract] [Full Text]
|
|
|
|
|
|
K. Ariizumi, G.-L. Shen, S. Shikano, R. Ritter III, P. Zukas, D. Edelbaum, A. Morita, and A. Takashima Cloning of a Second Dendritic Cell-associated C-type Lectin (Dectin-2) and Its Alternatively Spliced Isoforms J. Biol. Chem., April 14, 2000; 275(16): 11957 - 11963. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Danilkovitch, S. Donley, A. Skeel, and E. J. Leonard Two Independent Signaling Pathways Mediate the Antiapoptotic Action of Macrophage-Stimulating Protein on Epithelial Cells Mol. Cell. Biol., March 15, 2000; 20(6): 2218 - 2227. [Abstract] [Full Text]
|
|
|
|
|
|
A. Pacini, A. Quattrone, M. Denegri, C. Fiorillo, C. Nediani, S. R. y Cajal, and P. Nassi Transcriptional Down-regulation of Poly(ADP-ribose) Polymerase Gene Expression by E1A Binding to pRb Proteins Protects Murine Keratinocytes from Radiation-induced Apoptosis J. Biol. Chem., December 3, 1999; 274(49): 35107 - 35112. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Espada, M. Perez-Moreno, V. M.M. Braga, P. Rodriguez-Viciana, and A. Cano H-Ras Activation Promotes Cytoplasmic Accumulation and Phosphoinositide 3-Oh Kinase Association of {beta}-Catenin in Epidermal Keratinocytes J. Cell Biol., September 6, 1999; 146(5): 967 - 980. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Dong, Z. Chen, T. Kato, and C. Van Waes The Host Environment Promotes the Constitutive Activation of Nuclear Factor-{{kappa}}B and Proinflammatory Cytokine Expression during Metastatic Tumor Progression of Murine Squamous Cell Carcinoma Cancer Res., July 1, 1999; 59(14): 3495 - 3504. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Okimoto and H. Fan Moloney Murine Leukemia Virus Infects Cells of the Developing Hair Follicle after Neonatal Subcutaneous Inoculation in Mice J. Virol., March 1, 1999; 73(3): 2509 - 2516. [Abstract] [Full Text]
|
|
|
|
 |
|
 T. Fujimoto, M. Hayashi, M. Iwamoto, and Y. Ohno-Iwashita Crosslinked Plasmalemmal Cholesterol Is Sequestered to Caveolae: Analysis with a New Cytochemical Probe J. Histochem. Cytochem., September 1, 1997; 45(9): 1197 - 1206. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Tarutani, S. Itami, M. Okabe, M. Ikawa, T. Tezuka, K. Yoshikawa, T. Kinoshita, and J. Takeda Tissue-specific knockout of the mouse Pig-a gene reveals important roles for GPI-anchored proteins in skin development PNAS, July 8, 1997; 94(14): 7400 - 7405. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Bastaki, E. E. Nelli, P. Dell'Era, M. Rusnati, M. P. Molinari-Tosatti, S. Parolini, R. Auerbach, L. P. Ruco, L. Possati, and M. Presta Basic Fibroblast Growth Factor–Induced Angiogenic Phenotype in Mouse Endothelium : A Study of Aortic and Microvascular Endothelial Cell Lines Arterioscler Thromb Vasc Biol, March 1, 1997; 17(3): 454 - 464. [Abstract] [Full Text]
|
|
|
|
|
|
A. Utani, M. Nomizu, and Y. Yamada Fibulin-2 Binds to the Short Arms of Laminin-5 and Laminin-1 via Conserved Amino Acid Sequences J. Biol. Chem., January 31, 1997; 272(5): 2814 - 2820. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-F. Galliano, D. Aberdam, A. Aguzzi, J.-P. Ortonne, and G. Meneguzzi Cloning and Complete Primary Structure of the Mouse Laminin alpha3 Chain J. Biol. Chem., September 15, 1995; 270(37): 21820 - 21826. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R Ross, R Kleiz, and A B Reske-Kunz A method for rapid generation of competitive standard molecules for RT-PCR avoiding the problem of competitor/probe cross-reactions. Genome Res., June 1, 1995; 4(6): 371 - 375. [PDF]
|
|
|
|
|
|
A Blangy, F Vidal, F Cuzin, Y. Yang, K Boulukos, and M Rassoulzadegan CDEBP, a site-specific DNA-binding protein of the 'APP-like' family, is required during the early development of the mouse J. Cell Sci., January 2, 1995; 108(2): 675 - 683. [Abstract] [PDF]
|
|
|
|
|
|
T Fujimoto, A Miyawaki, and K Mikoshiba Inositol 1,4,5-trisphosphate receptor-like protein in plasmalemmal caveolae is linked to actin filaments J. Cell Sci., January 1, 1995; 108(1): 7 - 15. [Abstract] [PDF]
|
|
|
|
|
|
T Hashimoto, M Amagai, D. Parry, T. Dixon, S Tsukita, S Tsukita, K Miki, K Sakai, Y Inokuchi, J Kudoh, et al. Desmoyokin, a 680 kDa keratinocyte plasma membrane-associated protein, is homologous to the protein encoded by human gene AHNAK J. Cell Sci., January 6, 1993; 105(2): 275 - 286. [Abstract] [PDF]
|
|
|
|
|
|
K. Ariizumi, G.-L. Shen, S. Shikano, S. Xu, R. Ritter III, T. Kumamoto, D. Edelbaum, A. Morita, P. R. Bergstresser, and A. Takashima Identification of a Novel, Dendritic Cell-associated Molecule, Dectin-1, by Subtractive cDNA Cloning J. Biol. Chem., June 23, 2000; 275(26): 20157 - 20167. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Cancer Research | Clinical Cancer Research |
| Cancer Epidemiology Biomarkers & Prevention | Molecular Cancer Therapeutics |
| Molecular Cancer Research | Cancer Prevention Research |
| Cancer Prevention Journals Portal | Cancer Reviews Online |
| Annual Meeting Education Book | Meeting Abstracts Online |
