This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Findlay, D. M. Articles by Martin, T. J. Search for Related Content PubMed PubMed Citation Articles by Findlay, D. M. Articles by Martin, T. J.

Calcitonin and 1,25-Dihydroxyvitamin D₃ Receptors in Human Breast Cancer Cell Lines¹

Department of Medicine, University of Melbourne, Repatriation General Hospital, Heidelberg, Australia [D. M. F., V. P. M., J. A. E., R. J. F., J. M. M., I. M., T. J. M.], and Department of Immunology, Cancer Institute, Melbourne, [R. W.] Australia

Five human breast cancer cell lines (MCF 7, T 47D, BT 20, MDA 157, and MDA 231) and a human breast epithelial cell line (HBL 100) have been found to contain specific high-affinity receptors for 1,25-dihydroxyvitamin D₃. K_d values ranged from 0.6 to 2.0 x 10^-11 M and receptor concentration from 31 to 150 fmol/mg cytosol protein. Two of the breast cancer lines (MCF 7 and T 47D) contain specific high-affinity receptors for calcitonin and a calcitonin-responsive adenylate cyclase, which have been characterized with the aid of salmon, eel, and human calcitonins and in several substituted analogues of human calcitonin. The 1,25-dihydroxyvitamin D₃ receptor may reflect a normal property of the breast cell. Breast cancer cell lines provide a useful source of 1,25-dihydroxyvitamin D₃ receptors. Their coexistence with a calcitonin receptor and biological response in some breast cancers offers the opportunity to investigate new aspects of breast cancer endocrinology.

¹ Supported by the Anti-Cancer Council of Victoria, The National Health and Medical Research Council, and the Department of Veterans' Affairs.

² To whom requests for reprints should be addressed.

Received 4/16/80. Accepted 8/21/80.

This article has been cited by other articles:

				S. Chigurupati, T. Kulkarni, S. Thomas, and G. Shah Calcitonin Stimulates Multiple Stages of Angiogenesis by Directly Acting on Endothelial Cells Cancer Res., September 15, 2005; 65(18): 8519 - 8529. [Abstract] [Full Text] [PDF]

				G. M. Zinser and J. Welsh Accelerated Mammary Gland Development during Pregnancy and Delayed Postlactational Involution in Vitamin D3 Receptor Null Mice Mol. Endocrinol., September 1, 2004; 18(9): 2208 - 2223. [Abstract] [Full Text] [PDF]

				L. A. Tverberg, M. F. Gustafson, T. L. Scott, I. V. Arzumanova, E. R. Provost, A. W. Yan, and S. A. Rawie Induction of Calcitonin and Calcitonin Receptor Expression in Rat Mammary Tissue during Pregnancy Endocrinology, October 1, 2000; 141(10): 3696 - 3702. [Abstract] [Full Text] [PDF]

				A. Evdokiou, L.-J. Raggatt, G. J. Atkins, and D. M. Findlay Calcitonin Receptor-Mediated Growth Suppression of HEK-293 Cells Is Accompanied by Induction of p21WAF1/CIP1 and G2/M Arrest Mol. Endocrinol., October 1, 1999; 13(10): 1738 - 1750. [Abstract] [Full Text]

				C. Jagger, A. Gallagher, T. Chambers, and M. Pondel The Porcine Calcitonin Receptor Promoter Directs Expression of a Linked Reporter Gene in a Tissue and Developmental Specific Manner in Transgenic Mice Endocrinology, January 1, 1999; 140(1): 492 - 499. [Abstract] [Full Text]

				N. Morrison, J Shine, J. Fragonas, V Verkest, M. McMenemy, and J. Eisman 1,25-dihydroxyvitamin D-responsive element and glucocorticoid repression in the osteocalcin gene Science, December 1, 1989; 246(4934): 1158 - 1161. [Abstract] [PDF]