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Detection of Infectious Centers in C57BL/Ka Lymphoid Cell Populations Infected in Vitro by the Radiation Leukemia Virus¹

Cancer Biology Research Laboratory, Department of Radiology, Stanford University Medical Center, Stanford, California 94305

The cocultivation of nonproducer lymphoma cells derived from a radiation-induced lymphoma of the C57BL/Ka mouse with cultures of lymphoid cell populations from the thymus, spleen, and marrow of the same strain 48 hr after their infection by the C57BL/Ka leukemia viruses permits the detection of infectious centers in these cultures. A quantitative assay is described which allows the estimation in lymphoid cell subpopulations of the numbers of target cells susceptible to productive infection by the thymotropic and leukemogenic viruses of C57BL/Ka mice in vitro. This assay should greatly facilitate the identification and characterization of such target cells.

¹ This work was supported by Contract N01-CP-71052 from the National Cancer Institute, NIH, Department of Health, Education, and Welfare, and by gifts to the Jerry M. Tenney Memorial Fund for Cancer Research.

² Holder of Public Health Service International Fellowship 1F05 TW02546; on leave of absence from the Department of Pathology, University of Liège, Belgium, Laureate of the Braconnier-Lamarche Foundation, Liège, Belgium.

³ To whom requests for reprints should be addressed.

Received 6/29/79. Accepted 11/12/79.