This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ingraham, H. A. Articles by Goulian, M. Search for Related Content PubMed PubMed Citation Articles by Ingraham, H. A. Articles by Goulian, M.

Mechanism for Exclusion of 5-Fluorouracil from DNA¹

Department of Medicine, School of Medicine, University of California, San Diego, La Jolla, California 92093

5-Fluorodeoxyuridine 5'-triphosphate is hydrolyzed by the enzyme deoxyuridine triphosphate diphosphohydrolase (EC 3.6.1.23). Uracil-DNA glycosylase removes 5-fluorouracil (FUra) from FUra-containing DNA similar to its removal of uracil from uracil-containing DNA. The absence of FUra in DNA following exposure of cells to FUra can be explained by the activities of these two enzymes.

¹ This research was supported by USPHS Research Grant NIH-CA-11705.

² Recipient of USPHS Institutional Award HL-07107.

³ To whom requests for reprints should be addressed, at Department of Medicine, M-913, University of California, San Diego, La Jolla, Calif. 92093.

Received 8/14/79. Accepted 12/27/79.

This article has been cited by other articles:

				J. M. Carethers Review: Systemic treatment of advanced colorectal cancer: Tailoring therapy to the tumor Therapeutic Advances in Gastroenterology, July 1, 2008; 1(1): 33 - 42. [Abstract] [PDF]

				M. Meyers, M. W. Wagner, A. Mazurek, C. Schmutte, R. Fishel, and D. A. Boothman DNA Mismatch Repair-dependent Response to Fluoropyrimidine-generated Damage J. Biol. Chem., February 18, 2005; 280(7): 5516 - 5526. [Abstract] [Full Text] [PDF]

				S.-B. Lin, P. O. P. Ts'o, S.-K. Sun, K.-B. Choo, F.-Y. Yang, Y.-P. Lim, H.-L. Tsai, and L.-C. Au Inhibition of Thymidylate Synthase Activity by Antisense Oligodeoxynucleotide and Possible Role in Thymineless Treatment Mol. Pharmacol., September 1, 2001; 60(3): 474 - 479. [Abstract] [Full Text] [PDF]

				M. Meyers, M. W. Wagner, H.-S. Hwang, T. J. Kinsella, and D. A. Boothman Role of the hMLH1 DNA Mismatch Repair Protein in Fluoropyrimidine-mediated Cell Death and Cell Cycle Responses Cancer Res., July 1, 2001; 61(13): 5193 - 5201. [Abstract] [Full Text] [PDF]