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Longitudinal Karyotype and Genetic Signature Analysis of Cultured Human Colon Adenocarcinoma Cell Lines LS180 and LS174T¹

Departments of Biochemistry and Molecular Biology [L. P. R.] and Surgery [L. P. R., B. D. K.], The University of Texas Medical School, Houston, Texas 77030; Cook County Hospital, Chicago, Illinois 60612 [C. I. K., M. C.]; and The University of Texas System Cancer Center, Houston, Texas 77030 [M. J. S.]

Giemsa-banded chromosomes were analyzed at intervals during either 34 or 70 serial subcultivations of two cell lines, LS180 and LS174T, established from one primary human colon adenocarcinoma, and at passage 14 of autochthonous normal bowel cells, NB(LS174T). The cell lines were established and subcultured by either scraping or trypsin treatment of primary cultures; the scraped cell line was designated LS180, and the trypsin-dispersed cell line was named LS174T. Early passages of LS174T cells were composed mainly of 46,XX (38%) and 45,X (34%) karyotypes; LS180 cultures possessed cells with 46,XX (54%), 45,X (7.5%), and 47,XX+D (19.5%) chromosome modes. In both cell lines, the 45,X karyotype predominated in later subcultivations. After the fifth passage, all LS180 cells examined exhibited a translocation from the long arm of the X chromosome to the long arm of the No. 5 chromosome. Cultures from the patient's normal bowel mucosa and peripheral blood leukocytes had normal 46,XX karyotypes. Genetic signature analysis substantiated the common genetic origin of the cell lines, and we concluded that differences observed between LS180 and LS174T were not due to contamination with other cell lines. LS180 and LS174T represent closely related cell lines differing cytogenetically in a translocation.

¹ Supported by the USPHS Grant CA22370 from the National Cancer Institute through the National Large Bowel Cancer Project and by USPHS Grant CA23871. This report is one in a series of papers describing human colon adenocarcinoma cell lines (13, 14, 31, 36, 37). Preliminary reports were presented at the Annual Meetings of the Tissue Culture Association, June 5 to 8, 1978, Denver, Colo. (29), and June 10 to 14, 1979, Seattle, Wash. (30).

² To whom requests for reprints should be addressed, at Department of Surgery, Room 6240, P. O. Box 20708, Houston, Texas 77025.

Received 9/ 4/79. Accepted 1/29/80.

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