| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Department of Experimental Pathology, Roswell Park Memorial Institute, Buffalo, New York 14263 [G. P., J. D.], and Cancer Biology Program, National Cancer Institute, Frederick Cancer Research Center, Frederick, Maryland 21701 [I. R. H., I. J. F.]
New assay methods have been devised to quantitate tumor cell invasion of tissues of differing histological complexity maintained as organ cultures in vitro (chorioallantoic membrane of chicken, mouse urinary bladder, and canine blood vessel). In addition to quantitating tumor cell invasion, these methods also allow recovery of invasive cells for comparison with noninvasive cells. These methods have been used to select variant sublines from murine B16-F1 and B16-F10 melanoma lines that display significantly greater tissue-invasive abilities than the parent lines. B16 variant sublines selected in vitro for increased invasiveness through the bladder wall or vein also show a significant increase in their ability to form spontaneous and experimental metastases in vivo. In contrast, cells from the same parent cell line selected for increased invasiveness through the chorioallantoic membrane do not show significant alterations in metastatic behavior. We conclude that invasive variants can be isolated from the parent B16 tumor by several in vitro methods and that the level of expression of the invasive phenotype in vivo may be determined by the severity of the selection procedure in vitro.
1 Recipient of USPHS Research Grant CA 18260 and NIH Core Support Grant 17609 to Roswell Park Memorial Institute Cancer Cell Center.
2 To whom requests for reprints should be addressed.
3 Recipient of National Cancer Institute Contract NO1-CO-75380 with Litton Bionetics Inc.
Received 11/ 1/79. Accepted 2/ 7/80.
This article has been cited by other articles:
|
|
 |
|
  H. S. Kim, S. Kim-Schulze, D. W. Kim, and H. L. Kaufman Host Lymphodepletion Enhances the Therapeutic Activity of an Oncolytic Vaccinia Virus Expressing 4-1BB Ligand Cancer Res., November 1, 2009; 69(21): 8516 - 8525. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. HIRAKAWA, K. TANAKA, Y. TAKAYA, S. NAKAGAWA, Y. KATAOKA, Y. TAGAWA, and M. NIWA Development of a Novel Cell-based Assay to Evaluate the Malignant Potential of Cancer In Vitro Anticancer Res, July 1, 2009; 29(7): 2541 - 2545. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Zhang, F. Zhang, R. Tsan, and I. J. Fidler Transforming Growth Factor-{beta}2 Is a Molecular Determinant for Site-Specific Melanoma Metastasis in the Brain Cancer Res., February 1, 2009; 69(3): 828 - 835. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. F. Setiadi, M. D. David, R. P. Seipp, J. A. Hartikainen, R. Gopaul, and W. A. Jefferies Epigenetic Control of the Immune Escape Mechanisms in Malignant Carcinomas Mol. Cell. Biol., November 15, 2007; 27(22): 7886 - 7894. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Darmanin, J. Chen, S. Zhao, H. Cui, R. Shirkoohi, N. Kubo, Y. Kuge, N. Tamaki, K. Nakagawa, J.-i. Hamada, et al. All-trans Retinoic Acid Enhances Murine Dendritic Cell Migration to Draining Lymph Nodes via the Balance of Matrix Metalloproteinases and Their Inhibitors J. Immunol., October 1, 2007; 179(7): 4616 - 4625. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Jain, M. Zuka, J. Liu, S. Russell, J. Dent, J. A. Guerrero, J. Forsyth, B. Maruszak, T. K. Gartner, B. Felding-Habermann, et al. Platelet glycoprotein Ib{alpha} supports experimental lung metastasis PNAS, May 22, 2007; 104(21): 9024 - 9028. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. R. Langley and I. J. Fidler Tumor Cell-Organ Microenvironment Interactions in the Pathogenesis of Cancer Metastasis Endocr. Rev., May 1, 2007; 28(3): 297 - 321. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Carriere, R. Colisson, C. Jiguet-Jiglaire, E. Bellard, G. Bouche, T. Al Saati, F. Amalric, J.-P. Girard, and C. M'Rini Cancer Cells Regulate Lymphocyte Recruitment and Leukocyte-Endothelium Interactions in the Tumor-Draining Lymph Node Cancer Res., December 15, 2005; 65(24): 11639 - 11648. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. F. Setiadi, M. D. David, S. S. Chen, J. Hiscott, and W. A. Jefferies Identification of Mechanisms Underlying Transporter Associated with Antigen Processing Deficiency in Metastatic Murine Carcinomas Cancer Res., August 15, 2005; 65(16): 7485 - 7492. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Khanna and K. Hunter Modeling metastasis in vivo Carcinogenesis, March 1, 2005; 26(3): 513 - 523. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Hyoudou, M. Nishikawa, Y. Umeyama, Y. Kobayashi, F. Yamashita, and M. Hashida Inhibition of Metastatic Tumor Growth in Mouse Lung by Repeated Administration of Polyethylene Glycol-Conjugated Catalase: Quantitative Analysis with Firefly Luciferase-Expressing Melanoma Cells Clin. Cancer Res., November 15, 2004; 10(22): 7685 - 7691. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Hiraoka, S. Yamamoto, S. Otsuru, S. Nakai, K. Tamai, R. Morishita, T. Ogihara, and Y. Kaneda Enhanced Tumor-Specific Long-Term Immunity of Hemaggluttinating Virus of Japan-Mediated Dendritic Cell-Tumor Fused Cell Vaccination by Coadministration with CpG Oligodeoxynucleotides J. Immunol., October 1, 2004; 173(7): 4297 - 4307. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ito, N. Morita, D. Miura, Y.-i. Koma, T. R. Kataoka, H. Yamasaki, Y. Kitamura, Y. Kita, and H. Nojima A derivative of oleamide potently inhibits the spontaneous metastasis of mouse melanoma BL6 cells Carcinogenesis, October 1, 2004; 25(10): 2015 - 2022. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. E. Finkelstein, D. M. Heimann, C. A. Klebanoff, P. A. Antony, L. Gattinoni, C. S. Hinrichs, L. N. Hwang, D. C. Palmer, P. J. Spiess, D. R. Surman, et al. Bedside to bench and back again: how animal models are guiding the development of new immunotherapies for cancer J. Leukoc. Biol., August 1, 2004; 76(2): 333 - 337. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Ito, Y.-i. Koma, M. Sohda, K. Watabe, T. Nagano, Y. Misumi, H. Nojima, and Y. Kitamura Localization of the PP2A B56{gamma} Regulatory Subunit at the Golgi Complex: Possible Role in Vesicle Transport and Migration Am. J. Pathol., February 1, 2003; 162(2): 479 - 489. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ito, Y.-i. Koma, K. Watabe, T. Nagano, Y. Endo, H. Nojima, and Y. Kitamura A Truncated Isoform of the Protein Phosphatase 2A B56{gamma} Regulatory Subunit May Promote Genetic Instability and Cause Tumor Progression Am. J. Pathol., January 1, 2003; 162(1): 81 - 91. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.'i Nakamoto, A. Ito, K. Watabe, Y.-i. Koma, H. Asada, K. Yoshikawa, Y. Shinomura, Y. Matsuzawa, H. Nojima, and Y. Kitamura Increased Expression of a Nucleolar Nop5/Sik Family Member in Metastatic Melanoma Cells : Evidence for Its Role in Nucleolar Sizing and Function Am. J. Pathol., October 1, 2001; 159(4): 1363 - 1374. [Abstract] [Full Text]
|
|
|
|
 |
|
 K. HORINO, A. L. KINDEZELSKII, V. M. ELNER, B. A. HUGHES, and H. R. PETTY Tumor cell invasion of model 3-dimensional matrices: demonstration of migratory pathways, collagen disruption, and intercellular cooperation FASEB J, April 1, 2001; 15(6): 932 - 939. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Seliger, U. Wollscheid, F. Momburg, T. Blankenstein, and C. Huber Characterization of the Major Histocompatibility Complex Class I Deficiencies in B16 Melanoma Cells Cancer Res., February 1, 2001; 61(3): 1095 - 1099. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. G. Ostermeyer, B. T. Beckrich, K. A. Ivarson, K. E. Grove, and D. A. Brown Glycosphingolipids Are Not Essential for Formation of Detergent-resistant Membrane Rafts in Melanoma Cells. METHYL-beta -CYCLODEXTRIN DOES NOT AFFECT CELL SURFACE TRANSPORT OF A GPI-ANCHORED PROTEIN J. Biol. Chem., November 26, 1999; 274(48): 34459 - 34466. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Futami, M. Seno, M. Ueda, H. Tada, and H. Yamada Inhibition of cell growth by a fused protein of human ribonuclease 1 and human basic fibroblast growth factor Protein Eng. Des. Sel., November 1, 1999; 12(11): 1013 - 1019. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Sturmhoefel, K. Lee, G. S. Gray, J. Thomas, R. Zollner, M. O'Toole, H. Swiniarski, A. Dorner, and S. F. Wolf Potent Activity of Soluble B7-IgG Fusion Proteins in Therapy of Established Tumors and as Vaccine Adjuvant Cancer Res., October 1, 1999; 59(19): 4964 - 4972. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F Mitjans, D Sander, J Adan, A Sutter, J. Martinez, C. Jaggle, J. Moyano, H. Kreysch, J Piulats, and S. Goodman An anti-alpha v-integrin antibody that blocks integrin function inhibits the development of a human melanoma in nude mice J. Cell Sci., January 8, 1995; 108(8): 2825 - 2838. [Abstract] [PDF]
|
|
|
|
|
|
S. Yao and K Kurachi Implanted myoblasts not only fuse with myofibers but also survive as muscle precursor cells J. Cell Sci., January 8, 1993; 105(4): 957 - 963. [Abstract] [PDF]
|
|
|
|
 |
|
 M Nakajima, T Irimura, D Di Ferrante, N Di Ferrante, and G. Nicolson Heparan sulfate degradation: relation to tumor invasive and metastatic properties of mouse B16 melanoma sublines Science, May 6, 1983; 220(4597): 611 - 613. [Abstract] [PDF]
|
|
|
|
|
|
I. Fidler and I. Hart Biological diversity in metastatic neoplasms: origins and implications Science, September 10, 1982; 217(4564): 998 - 1003. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Cancer Research | Clinical Cancer Research |
| Cancer Epidemiology Biomarkers & Prevention | Molecular Cancer Therapeutics |
| Molecular Cancer Research | Cancer Prevention Research |
| Cancer Prevention Journals Portal | Cancer Reviews Online |
| Annual Meeting Education Book | Meeting Abstracts Online |
