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Cellular Potentials of Normal and Cancerous Fibroblasts and Hepatocytes

Department of Anatomy, University of Southern California, Los Angeles, California 90033 [R. B.], and Department of Anatomy, University of Texas Health Science Center at San Antonio, San Antonio, Texas 78284 [I. L. C.]

Several lines of investigation point to differences in electrical properties between normal and cancerous cells. Several tumor lines have low-resting membrane potentials. A few comparisons have been made between normal and tumor cells within the same tissue cell type. This study compares the cellular or transmembrane potential of hepatocytes and fibroblasts in both normal and tumor cells.

High-impedance micropipets were used to record intracellularly in vivo in Buffalo rat hepatocytes and Morris 7777 hepatoma cells, as well as A/J mouse corneal fibroblasts and poorly differentiated fibrosarcoma cells.

Rat hepatocytes had a mean membrane potential of -37.1 ± 4.3 (S.D.) mV compared to -19.8 ± 7.1 mV in the hepatoma cells. Mouse corneal fibroblasts measured -42.5 ± 5.4 mV, while cells of mouse fibrosarcoma were -14.3 ± 5.4 mV. The membrane potentials of the tumor cells were lower in both instances than in their normal counterpart (statistically significant at p = 0.001 for both tissue cell types).

This supports the notion that lower cellular or membrane potentials may play a significant role in the altered physiology of the tumor cell.

¹ To whom requests for reprints should be addressed, at the Department of Anatomy, University of Southern California School of Medicine, 2025 Zonal Avenue, Los Angeles, Calif. 90033.

Received 9/19/79. Accepted 2/26/80.

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