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Ultraviolet Light-induced Transformation of Human Cells to Anchorage-independent Growth¹

Biology Department, Brookhaven National Laboratory, Upton, New York 11973

We have developed a system for ultraviolet light (UV) transformation of human embryonic cells to anchorage-independent growth. The procedure involves multiple UV irradiations, post irradiation growth, and plating in soft agar. Transformants are obtained at frequencies from 1 to 80 per 10⁵ cells at UV exposures to 25 J/sq m. The resulting transformants can be subcultured on solid surfaces. The cells show crisscrossing and piling up; they reach 2- to 5-fold higher saturation densities than the parental cells. Some subcultures show increased plating efficiency in soft agar and increased life span. The susceptibility of the UV transformation process to apparent photoenzymatic reversal implies that pyrimidine dimers play a role in its induction.

¹ This research was supported by Grants CA 23096 from the National Cancer Institute and NP-154B from the American Cancer Society and by the United States Department of Energy.

² Recipient of a Research Career Development Award (CA 00466) from the National Cancer Institute. To whom requests for reprints should be addressed.

³ This investigation was begun while these authors were at the Department of Molecular Biology, University of California, Irvine, Calif.

Received 7/30/79. Accepted 2/28/80.

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