This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Banerjee, S. Articles by Oruambo, F. I. Search for Related Content PubMed PubMed Citation Articles by Banerjee, S. Articles by Oruambo, F. I.

Microsome-mediated Covalent Binding of 1,2-Dichloroethane to Lung Microsomal Protein and Salmon Sperm DNA¹

Laboratory of Organic Chemistry and Carcinogenesis, Institute of Environmental Medicine, New York University Medical Center, New York, New York 10016

In order to determine whether the covalent binding of the carcinogen 1,2-dichloroethane to macromolecules is dependent on microsomes or cytosol, microsomes and cytosol from lungs of C57BL/6 x C3H/He F₁ (hereafter called B6C3F₁) mice and Osborne-Mendel rats were incubated with [1,2-¹⁴C]dichloroethane and salmon sperm DNA. 1,2-Dichlorothane binds covalently to microsomal protein and DNA only in the presence of microsomes, whereas cytosol has insignificant metabolic activation. The binding to macromolecules was significantly higher in the presence of native microsomes than denatured microsomes. The interaction of 1,2-dichloroethane with DNA was enhanced following pretreatment of the animals with phenobarbital and 3-methylcholanthrene. On the other hand, glutathione reduced the binding. The binding of 1,2-dichloroethane to lung microsomal protein of B6C3F₁ mice and to DNA was three and five times higher, respectively, than that of Osborne-Mendel rat lung microsomal proteins. 1,2-Dichloroethane interacts 85% and 100% more with protein and DNA, respectively, in the presence of microsomes obtained from lung than from liver of B6C3F₁ mice. These results suggest a correlation between the microsomally mediated binding and species and organ susceptibility to 1,2-dichloroethane-induced tumorigenesis.

¹ Supported by National Science Foundation Grant PFR76-10656 and by USPHS Grants ES-00260 and CA-13343.

Received 1/23/80. Accepted 3/27/80.