This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bevins, C. L. Articles by Bashirelahi, N. Search for Related Content PubMed PubMed Citation Articles by Bevins, C. L. Articles by Bashirelahi, N.

Stabilization of 8S Progesterone Receptor from Human Prostate in the Presence of Molybdate Ion

Department of Biochemistry, School of Dentistry [C. L. B., N. B.], and the Department of Surgery (Urology), School of Medicine [N. B.], University of Maryland, Baltimore, Maryland 21201

In the presence of 10 mM molybdate ion, we are able to detect the appearance of a [6,7-³H]-17,21-dimethyl-19-nor-4,9-pregnadiene-3,20-dione- ([³H]R5020) binding moiety in human prostatic cytosol which sedimented at approximately 8S in a glycerol density gradient. The specifically bound [³H]-R5020 was displaced by progesterone, tiramcinolone acetonide, and R5020 but not by cortisol, dihydrotestosterone, 17ß-estradiol, or diethylstilbestrol. Specific binding of [³H]R5020 in the presence of molybdate ion was shown to saturate at 7 x 10^-10 M (n = 64 fmol/mg of protein). The optimum concentration of molybdate ion for enhancing specific binding of [³H]-R5020 was determined to be between 7 and 20 mM. Molybdate ion was also effective in stabilizing the 8S R5020-binding moiety in a preincubation for 16 hr at 0° in the absence of added steroid.

¹ This work was undertaken in partial fulfillment of research requirements for the University-wide Ph.D. program in biochemistry at the University of Maryland.

² To whom requests for reprints should be addressed.

Received 10/31/79. Accepted 3/20/80.

This article has been cited by other articles:

				W. B. Pratt and D. O. Toft Steroid Receptor Interactions with Heat Shock Protein and Immunophilin Chaperones Endocr. Rev., June 1, 1997; 18(3): 306 - 360. [Abstract] [Full Text]