This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Pallante, S. L. Articles by Colvin, M. Search for Related Content PubMed PubMed Citation Articles by Pallante, S. L. Articles by Colvin, M.

Quantitation by Gas Chromatography-Chemical Ionization-Mass Spectrometry of Phenylalanine Mustard in Plasma of Patients¹

Department of Pharmacology and Experimental Therapeutics [S. L. P., C. F., M. A.], Department of Obstetrics and Gynecology [N. B. R.], and the Oncology Center [R. G. M., R. B. B., M. C.], The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205

An unambiguous and sensitive method based on gas chromatography-chemical ionization-mass spectrometry has been developed to quantitate L-phenylalanine mustard and has been applied to measure levels in plasma of five patients receiving 0.15 to 0.25 mg/kg (10 to 17 mg) of the drug p.o. Peak plasma levels of 50 to 190 ng/ml were found to occur between 0.7 and 2.3 hr after ingestion. The time for the plasma level to fall to one-half of the peak value varied from 0.6 to 3 hr, and very low levels (<2 ng/ml) were present by 24 hr.

¹ This research was supported by USPHS NIH Grants CA-16783, CA-00103, GM-21248, and CA-00673. These data were presented in part at the 27th Annual Conference on Mass Spectrometry and Allied Topics, Seattle, Wash., June 3 to 8, 1979.

² To whom requests for reprints should be addressed, at the Department of Pharmacology and Experimental Therapeutics, 725 North Wolfe Street, Baltimore, Md., 21205.

Received 10/15/79. Accepted 3/21/80.