This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Kunz, B. A. Articles by Haynes, R. H. Search for Related Content PubMed PubMed Citation Articles by Kunz, B. A. Articles by Haynes, R. H.

Effect of Tumor Promoters on Ultraviolet Light-induced Mutation and Mitotic Recombination in Saccharomyces cerevisiae¹

Department of Biology, York University, Toronto, Ontario, Canada M3J 1P3 [B.A.K., R.H.H.], and Ephraim McDowell Community Cancer Network and Division of Experimental Pathology, University of Kentucky, Lexington, Kentucky 40506 [M.A.H.]

Recently, it has been suggested that mitotic recombination is involved in tumor promotion. On this basis, one might expect tumor promoters to be recombinagenic. D7 is a diploid strain of yeast in which both mutation and mitotic recombination can be measured. We have used this strain to assay the known tumor promoters, iodoacetate, anthralin, and 12-O-tetradecanoylphorbol-13-acetate, and the cocarcinogen, catechol, for mutagenicity, recombinagenicity, and the ability to enhance ultraviolet light (UV)-induced genetic events. In the absence of preirradiation with UV, iodoacetate was found to be recombinagenic whereas catechol was mutagenic; however, in both cases, the effects were small. Iodoacetate, anthralin, and catechol potentiated UV-induced mitotic crossing-over, aberrant colony formation, and mutation, while catechol also increased UV-induced gene conversion. We were unable to detect any mutagenic or recombinagenic effect of 12-O-tetradecanoylphorbol-13-acetate in either whole cells or spheroplasts. Our results do not indicate any consistent correlation between tumor-promoting activity and the ability of an agent to induce mitotic recombination in yeast. However, the ability to potentiate UV-induced mutation and mitotic recombination may reflect the cocarcinogenic activity of certain promoters.

¹ Supported by grants from the National Research Council of Canada and the Natural Sciences and Engineering Research Council of Canada.

² To whom requests for reprints should be addressed.

Received 11/15/79. Accepted 4/ 8/80.