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Radiolabeling of Affinity-purified Goat Anti-Carcinoembryonic Antigen Immunoglobulin G with Technetium-99m¹

Nuclear Medicine Service, Veterans Administration Medical Center, Lexington 40507 [W. A. P., F. H. D.], and Division of Experimental Pathology, Department of Pathology, University of Kentucky Medical Center, and Ephraim McDowell Community Cancer Network, Inc., Lexington, Kentucky 40536 [S. J. B., D. M. G.]

Affinity-purified goat anti-carcinoembryonic antigen immunoglobulin G was labeled with ^99mTc, utilizing stannous tartrate as the reducing agent. The radiochemical yield of labeled antibody ranged from 18 to 37% and was inversely proportional to the initial quantity of pertechnetate ion used. Yields up to 60% could be obtained using small amounts of pertechnetate. the ^99mTc-labeled anti-carcinoembryonic antigen immunoglobulin G preparations had a specific activity range of 1 to 6 µCi/µg protein and had 60% or greater immunoreactivity. Human serum albumin and goat immunoglobulin G were used as model systems to evaluate labeling parameters and analytical methods.

¹ Presented at the UICC Workshop on Radioimmunodetection of Cancer, July 19 to 21, 1979, Lexington, Ky. Supported in part by NIH Grants CA-17742 and CA-25584, NIH Contract NIC-N01-CB-64011-35, and by a grant from the Veterans Administration.

² To whom requests for reprints should be addressed.