This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Farrelly, J. G. Search for Related Content PubMed PubMed Citation Articles by Farrelly, J. G.

A New Assay for the Microsomal Metabolism of Nitrosamines¹

Chemical Carcinogenesis Program, Frederick Cancer Research Center, Frederick, Maryland 21701

This report describes a new general assay for the microsomal oxidative dealkylation of nitrosamines. After precipitation of the microsomal proteins, aldehydes formed from nitrosamines are quantitated by high-pressure liquid chromatography as their 2,4-dinitrophenylhydrazones. This novel assay method offers some distinct advantages over the commonly used Nash reagent assay. Dimethylnitrosamine, diethylnitrosamine, and methylethylnitrosamine were metabolized by microsomes from noninduced male Fischer rat liver, and the aldehydes produced by these reactions were examined. Two distinct kinetic lines were observed for reactions containing dimethylnitrosamine or diethylnitrosamine. Similarly, two K_m's were observed for the production of both formaldehyde and acetaldehyde produced by the metabolism of methylethylnitrosamine.

¹ This work was sponsored by the National Cancer Institute under Contract NO1-CO-75380 with Litton Bionetics, Inc.

Received 9/21/79. Accepted 6/11/80.

This article has been cited by other articles:

				J. F. Teiber and P. F. Hollenberg Identification of the human liver microsomal cytochrome P450s involved in the metabolism of N-nitrosodi-n-propylamine Carcinogenesis, August 1, 2000; 21(8): 1559 - 1566. [Abstract] [Full Text] [PDF]