This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Ziboh, V. A. Articles by Kursunoglu, I. Search for Related Content PubMed PubMed Citation Articles by Ziboh, V. A. Articles by Kursunoglu, I.

Arachidonic Acid Metabolism by Rat Chloroleukemia Cells in Culture¹

Departments of Dermatology and Medicine, University of Miami School of Medicine, Miami, Florida 33101

Rat chloroleukemic cells as well as turpentine-induced rat hyperplastic bone marrow cells in culture medium incorporated approximately 63% of [1-¹⁴C]arachidonic acid added to the medium into their cellular membrane phospholipids. Separation of the cellular phospholipid fraction by thin-layer chromatography revealed that most of the [¹⁴C]arachidonic acid was incorporated into phosphatidylcholine (48%) and phosphatidylethanolamine (12%). The rest of the 3% radioactivity was distributed in sphingomyelin, phosphatidylserine, phosphoinositides, plasmalogen, and phosphatidic acid.

Metabolism of [¹⁴C]arachidonic acid by the chloroleukemic cells and hyperplastic marrow cells during a six-hr quiescent period in culture resulted in a marked transformation of the labeled fatty acid into prostaglandin D₂. Biosynthesis of prostaglandin D₂ was statistically (p < 0.005) greater in the chloroleukemic cells than in the hyperplastic marrow cells during this nonproliferating phase of both cell types. Biosynthesis of prostaglandin F₂ (PGF₂) by both cells was minimal during this period. However, 24 hr after both cells had been incubated in culture, the chloroleukemic cells exhibited an almost linear increase in growth, whereas the hyperplastic marrow cells remained quiescent. Biosynthesis of [¹⁴C]prostaglandin D₂ by both cell types decreased markedly when compared to the six-hr period, whereas the biosynthesis of [¹⁴C]PGF₂ by the rapidly proliferating chloroleukemic cells increased markedly. No significant alteration in PGF₂ biosynthesis occurred in the quiescent hyperplastic marrow cells. For instance, a statistically significant (p < 0.001) amount of extracted ¹⁴C in the prostaglandin fraction was associated with PGF₂ formed by the chloroleukemic cells when compared to the hyperplastic marrow cells. These results suggest that the increased formation of PGF₂ associated with the rapidly proliferating chloroleukemic cells in culture may reflect, at least in part, a property of the rapidly growing cells. Whether these results relate to the abnormal hyperproliferative nature of these cells remains to be determined.

¹ Supported in part by Grants AM14941, CA 19182, and IN-515 from the American Cancer Society.

² To whom requests for reprints should be addressed, at Department of Dermatology, R-117, University of Miami School of Medicine, P. O. Box 016960, Miami, Fla. 33101.

³ Howard Hughes Investigator.

Received 6/27/79. Accepted 9/26/80.