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Intratumor Maturational Heterogeneity within the Murine Myeloma MOPC-315¹

Division of Biology and Biomedical Sciences, Washington University School of Medicine, St. Louis, Missouri 63110

The murine myeloma MOPC-315 secretes a paraprotein which binds dinitrophenylated compounds. Maturational subsets which mimic normal B-cell differentiation were shown to exist within this monoclonal neoplasm. The maturational subsets were defined by an in vivo stem cell activity (plasmacytoma colony-forming unit-spleen), DNA synthesis ([³H]thymidine incorporation), membrane-bound paraprotein, and cells secreting the MOPC-315 paraprotein. Velocity sedimentation at unit gravity separated cells enriched for secreting the MOPC-315 protein (14.6 mm/hr) from the plasmacytoma colony-forming unit-spleen enriched population (9.3 mm/hr). An in vivo sequential analysis of the appearance of tumor (i.v. challenge) in the spleen of BALB/c hosts did not reveal any discordant appearance of these same maturational subsets with respect to time. The MOPC-315 myeloma contains maturational subsets that mimic normal B-cell differentiation and apparently differentiates during a very early stage of its evolution within the host.

¹ Work performed while the author was a graduate student in the Department of Pathology, Washington University School of Medicine, St. Louis, Missouri.

² Present address: Immunobiology Laboratories, Department of Pathology, University of New Mexico School of Medicine, Albuquerque, N. M. 87131.

Received 11/20/79. Accepted 10/ 8/80.