This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by LeGrue, S. J. Articles by Kahan, B. D. Search for Related Content PubMed PubMed Citation Articles by LeGrue, S. J. Articles by Kahan, B. D.

Immunobiological Properties of 1-Butanol-extracted Cell Surface Antigens¹

Departments of Biochemistry and Surgery, The University of Texas Health Science Center, Houston, Texas 77030, and The University of Texas Science Park, Smithville, Texas 78957 [J. P. A.]

Extracts of viable 3-methylcholanthrene-induced murine sarcoma cells (MCA-F and MCA-2A) prepared using single-phase (2.5%) 1-butanol significantly retarded the outgrowth of the homotypic, but not the heterotypic, tumor of syngeneic C3H/HeJ mice. Butanol extracts specifically evoked a delayed hypersensitivity response in tumor-immune syngeneic mice, but not in alloimmune DBA/2J mice. Crude butanol extracts of MCA-F cells did not contain alloantigenic activity, as shown by their inability to block H-2 or la-specific antibodies in a complement-dependent cytotoxicity assay. Absorption of these same allospecific reagents with untreated or with butanol-extracted cells indicated that H-2 antigens remain associated with the cell surface during extraction. Thus, butanol appears to release tumor antigens, but not alloantigens, from the cell surface.

¹ This work was supported by American Cancer Society Grant IM-62-F and by USPHS Grant CA-26321, awarded by the National Cancer Institute, Department of Health and Human Services.

² Recipient of a National Research Service Award CA-06326 from the National Cancer Institute, Department of Health and Human Services. To whom requests for reprints should be addressed.

Received 4/13/81. Accepted 7/14/81.

This article has been cited by other articles:

				T. Ariyasu, O. Ike, S. Hitomi, H. Wada, Y. Okumura, and J. Sunamoto Sensitization of Nude Mice Using Direct Liposome Transfer of Tumor Cell Antigens Journal of Bioactive and Compatible Polymers, July 1, 1996; 11(3): 191 - 202. [Abstract]