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Tissue and Urinary Glycosaminoglycan Patterns Associated with a Fast, an Intermediate, and a Slow-growing Morris Hepatoma¹

Cancer Center, University of Louisville, Louisville, Kentucky 40201 [C. E. K., E. E. D., J. K., K. L. C., R. W.], and the Department of Biochemistry, Cancer Research Unit, College of Medicine, Howard University, Washington, D. C. [H. P. M.]

The purpose of this investigation was to evaluate the glycosaminoglycans (GAG's) in different behavioral-histological types of i.m.-transplanted hepatomas and in the liver and urine of animals bearing these tumors. Groups of 10 Buffalo rats carrying fast-growing (7777), intermediate (5123tc), and slow-growing (9618A) Morris hepatomas were studied as the tumors reached 3 cm. Urinary and tissue GAG's were isolated by proteolysis, separated as cetylpyridinium complexes, and measured as uronic acid. The GAG's were further purified using anion-exchange chromatography and characterized with mucopolysaccharidases. Tissue GAG's were also evaluated histochemically using Alcian blue staining and mucopolysac-charidases. Tissue from fast-growing, intermediate, and slow-growing tumors exhibited greater GAG levels than did normal liver in the hyaluronic acid (0.4 M NaCl-soluble) fraction and in the chondroitin sulfate-heparan sulfate (1.2 M NaCl-soluble) fraction. The livers of tumor-bearing animals exhibited GAG levels similar to those of normal liver. Increased urinary GAG excretion was appreciated in animals bearing Tumors 5123tc and 9618A but not in those bearing Tumor 7777.

¹ Supported by American Cancer Society Grant IN-111B, by a grant from the Manufacturing Chemists Association, and in part by USPHS Grants CA 10729 and CA 246201.

² Present address: Biological Sciences, Murray State University, Murray, Ky. 42071. To whom requests for reprints should be addressed.

Received 4/11/80. Accepted 10/24/80.

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