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Lysis of Cultured Human Melanoma M10 Cells by Polyclonal Xenoantibodies to Melanoma-associated Antigens¹

Department of Molecular Immunology, Scripps Clinic and Research Foundation, La Jolla, California 92037

Cultured human melanoma cells M10 harvested from cultures in different phases of their growth show significant changes in the expression of melanoma-associated antigens (MAA), but they do not vary in susceptibility to lysis mediated by anti-MAA xenoantisera and effected by complement or lymphoid cells. Furthermore, melanoma cells M10 showed a significant increase in susceptibility to immune lysis following treatment with puromycin at doses that do not effect the expression of MAA. The lack of correlation between MAA density and susceptibility to immune lysis supports the contention that, under the experimental conditions used, cellular properties play a major role in the outcome of immune attack.

¹ Supported by USPHS Grants Al 13154, CA 16069, and CA 16071. This is Publication 1794 from Scripps Clinic and Research Foundation.

² Fellow of the Leukemia Society of America.

³ Recipient of a Research Career Development Award from the National Cancer Institute.

⁴ Recipient of an American Heart Association Established Investigatorship Award. To whom requests for reprints should be addressed.

Received 12/10/79. Accepted 11/ 4/80.