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[Cancer Research 41, 1351-1357, April 1, 1981]
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Identification, Purification, and Radioimmunoassay of NB/70K, a Human Ovarian Tumor-associated Antigen1

Suzanne Knauf and Gerald I. Urbach2

Department of Obstetrics and Gynaecology, University of Toronto, The Wellesley Hospital, Toronto, Ontario, Canada

NB/70K, a tumor-associated antigen of human ovarian epithelial tumor Fraction OCA, has been purified and identified as a glycoprotein which is stable in 0.6 M perchloric acid, binds to concanavalin A, and migrates electrophoretically with {alpha}-like mobility in barbital-buffered agarose at pH 8.6. NB/70K does not appear to contain normal serum, normal ovary, normal lung, or carcinoembryonic antigen-like cross-reacting antigenic determinants as measured by radioimmunoassay. NB/70K has been purified from ovarian antigen Fraction OCA by chromatography on {gamma}-globulin coupled to Sepharose 4B and by elution from acrylamide gels. NB/70K migrates as a single band with an apparent molecular weight of 70,000 in sodium dodecyl sulfate:acrylamide gel electrophoresis.

A rabbit antibody raised against NB/70K was able to precipitate a polypeptide with a molecular weight of 70,000 as visualized by autoradiography of sodium dodecyl sulfate:acrylamide gels. A radioimmunoassay has been developed for measuring NB/70K activity, using Staphylococcus aureus protein A as a precipitating agent.

1 Supported by Ontario Cancer Treatment and Research Foundation Project 324.

2 To whom requests for reprints should be addressed, at Suite 805, 600 Sherbourne Street, Toronto, Ontario, Canada M4X 1W4.

Received 6/23/80. Accepted 12/31/80.




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J. A. Roberts
Searching for a Biomarker for Ovarian Cancer
JAMA, August 26, 1998; 280(8): 739 - 739.
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HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Cell Growth & Differentiation
Copyright © 1981 by the American Association for Cancer Research.