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Effect of Estrogen on Metabolism and Enzyme Activities of Mammary Adenocarcinoma R3230AC in Fischer Rats¹

Bruce Lyon Memorial Research Laboratory, Children's Hospital Medical Center, Oakland, California 94609 [S. A., L. A. H., F. N. H., C. Y. L.], and Department of Biochemistry, University of Rochester Medical Center, Rochester, New York 14642 [R. H.]

Metabolic characteristics and enzyme activities are reported for a variant line of the R3230AC tumor, a long-established, transplantable mammary adenocarcinoma in the Fischer rat. Lipogenic enzyme activities from the variant tumor are generally lower than those reported for early generations of this tumor. In addition, the proportion of the medium-chain-length fatty acids in the lipids of this variant line is lower.

In the variant tumor, estrogen treatment of host animals results in a reduction of fatty acid synthesis from both acetate and glucose. The response of the variant tumor to estrogen treatment is similar to that for the normal gland in a virgin rat in that lipogenic enzyme activities are lowered in both normal and neoplastic tissues. These enzymatic activities in early generations of tumor R3230AC were similar to those observed in mammary glands from lactating rather than virgin rats. Since the variant tumor still responds to estrogen treatment by a reduced growth rate, we have inferred that, although it undergoes the same structural differentiation observed in earlier generations, the variant tumor line no longer shows certain biochemical changes associated with the lactational state in the normal gland.

¹ This investigation was supported by Grants CA 11736 and CA 16660 awarded by the National Cancer Institute, NIH, Department of Health, Education, and Welfare.

² To whom requests for reprints should be addressed, at Children's Hospital Medical Center, 51st and Grove Sts., Oakland, Calif. 94609.

Received 10/14/80. Accepted 1/13/81.