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Discordant Synthesis and Secretion of Human Chorionic Gonadotropin and Subunits by Cervical Carcinoma Cells¹

Departments of Gynecology and Obstetrics and of Biochemistry, Medical College of Wisconsin, Wauwatosa, Wisconsin 53226 [L. A. C., R. O. H.], and Department of Obstetrics and Gynecology, University of Louisville, School of Medicine, Louisville, Kentucky 40202 [C. V. R.]

Epidermoid carcinoma of the cervix cell lines DoT and CaSki were examined for the production of human chorionic gonadotropin (HCG) and its subunits (HCG- and HCG-ß). Additionally, the effect of butyrate and other regulatory agents on synthesis and secretion was investigated. Media and cell lysates were analyzed by radioimmunoassay for HCG and HCG-ß and by radioreceptor assay for intact HCG. Although DoT and CaSki cells secreted more HCG-ß than HCG, the cell lysates contained only one-half as much HCG-ß as HCG. This raises the possibility that HCG- synthesis may be limiting to the formation of intact HCG in DoT and CaSki cell lines. Our results also indicate discordance in HCG and HCG-ß production in response to butyrate. Whereas the production of HCG-ß was increased by 5 mM sodium butyrate, the synthesis and secretion of intact hCG were decreased. These results support the concept of independent regulation of - and ß-subunit synthesis and secretion.

¹ This work was sponsored by Grant CA23357, awarded by the National Cancer Institute, Department of Health, Education, and Welfare.

² To whom requests for reprints should be addressed.

Received 5/ 8/80. Accepted 1/22/81.

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