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Induction of Anchorage-independent Growth in Human Fibroblasts by Propane Sultone¹

Carcinogenesis Laboratory—Fee Hall, Departments of Microbiology and Biochemistry, Michigan State University, East Lansing, Michigan 48824

We have demonstrated a dose-dependent increase in the frequency of diploid human cells capable of anchorage-independent (AI) growth after treatment with the carcinogen propane sultone, followed by exponential growth to allow full expression of this phenotype (8 to 13 population doublings). Exposure to these same concentrations of propane sultone also resulted in a dose-dependent increase in the frequency of 6-thioguanine-resistant cells in the population. Procedures such as synchronization of cells and treatment just after the onset of DNA synthesis or the use of special selective medium were not essential for this induction. A very low frequency of cells with the AI phenotype was found in the control population (background). Cells which exhibited the AI phenotype spontaneously or after carcinogen treatment retained the characteristic over as many generations as tested (>13). The data suggest that AI growth is the result of a mutational event.

¹ Supported in part by Department of Health and Human Services Grant CA 21289 from the National Cancer Institute and by a grant from the Elsa U. Pardee Foundation.

² To whom requests for reprints should be addressed.

Received 9/15/80. Accepted 1/22/81.

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