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Action Spectra for Ultraviolet Light-induced Transformation of Human Cells to Anchorage-independent Growth¹

Biology Department, Brookhaven National Laboratory, Upton, New York 11973

We have determined action spectra for transformation of human embryonic skin and muscle fibroblasts to anchorage-independent growth. Tests under our experimental conditions indicate that reciprocity holds for photon rate and exposure time and that all the dose-effect curves in the wavelength range of 248 to 297 nm are similar. These data can be used to construct action spectra with a maximum at about 265 nm, which do not implicate moieties other than nucleic acids as absorbers in the transformation process.

¹ This research was supported by Grants CA23096 from the National Cancer Institute and NP-154B from the American Cancer Society and by the United States Department of Energy.

² Recipient of Research Career Development Award CA00466 from the National Cancer Institute. To whom requests for reprints should be addressed.

³ This investigation was begun while these authors were at the Department of Molecular Biology, University of California, Irvine, Calif.

⁴ Recipient of Research Career Development Award CA00465 from the National Cancer Institute. This investigation was begun while this author was at the Department of Physiology, California College of Medicine, University of California, Irvine, Calif.

Received 8/ 9/80. Accepted 3/ 5/81.