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Reduced -Cystathionase Protein Content in Human Malignant Leukemia Cell Lines as Measured by Immunoassay with Monoclonal Antibody¹

Department of Medicine [L. M. G., C. G. S.], and The Eleanor Roosevelt Institute for Cancer Research [A. E.], University of Colorado Health Sciences Center, Denver, Colorado 80262

A murine hybrid cell line has been produced which secretes immunoglobulin G₂b with specificity for human -cystathionase (EC 4.2.1.15). The antibody has been iodinated and used in combination with quantitative immunoelectrophoresis in an assay which is capable of detecting as little as 1.5 ng enzyme protein. Human lymphoblastic leukemia cell lines CEM and Laz-221 contain undetectable enzyme protein, corresponding to their behavior as cysteine auxotrophs. In contrast, nonmalignant lymphoblastoid lines contain easily detectable enzyme protein which correlates with their behavior as cysteine prototrophs. Other malignant leukocyte cell lines contained detectable but variable amounts of enzyme protein, suggesting that the enzyme may be a useful marker of cellular differentiation.

¹ This work was supported in part by gifts from the Colorado Chapter of the Veterans of Foreign Wars and the Tad Beck Foundation. We also received support from American Cancer Society Grant IN5S.

² Junior Faculty Clinical Fellow of the American Cancer Society. To whom requests for reprints should be addressed.

Received 11/ 3/80. Accepted 2/27/81.

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