This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Fisher, M. S. Articles by Cifone, M. A. Search for Related Content PubMed PubMed Citation Articles by Fisher, M. S. Articles by Cifone, M. A.

Enhanced Metastatic Potential of Murine Fibrosarcomas Treated in Vitro with Ultraviolet Radiation¹

Cancer Biology Program [M. S. F.], and Cancer Metastasis and Treatment Laboratory [M. A. C.], National Cancer Institute Frederick Cancer Research Center, Frederick, Maryland 21701

The purpose of this study was to determine whether repeated treatment of tumor cells in vitro with mutagenic doses of ultraviolet (UV) radiation could influence the metastatic behavior of these cells in vivo. Three cloned lines of UV-2237, a fibrosarcoma induced in a C3H^- mouse by chronic irradiation with UV, and SF-19, a spontaneous C3H^- fibrosarcoma, were grown in culture. These cell lines varied from low to high metastatic potential as determined by in vivo tests. The cultures were exposed to UV radiation from an FS40 sunlamp at a dose that killed 40% of the cells. These UV radiation exposures were repeated at 3- to 5-day intervals for a total of 5 treatments. The mutation frequency was analyzed by monitoring the appearance of ouabain-resistant colonies following UV irradiation. With all four tumor lines, the frequency of conversion to ouabain resistance was increased more than 10-fold. Tumor cells given 5 UV radiation treatments and control cultures carried in parallel without exposure to UV radiation were tested for metastatic potential in an in vivo lung colony assay. Cell lines treated in vitro with UV radiation produced more experimental metastases than the counterpart unirradiated cultures. We conclude that, in all four tumor lines, exposure of tumorigenic cells to mutagenic doses of UV radiation can alter their biological behavior and that this may contribute to the progression of tumors from low to high metastatic capability.

¹ Research sponsored by the National Cancer Institute under Contract NO1-CO-75380 with Litton Bionetics, Inc.

² To whom requests for reprints should be addressed, at Frederick Cancer Research Center, P. O. Box B, Frederick, Md 21701.

Received 10/20/80. Accepted 4/22/81.

This article has been cited by other articles:

				I. Fidler and I. Hart Biological diversity in metastatic neoplasms: origins and implications Science, September 10, 1982; 217(4564): 998 - 1003. [Abstract] [PDF]