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Immunochemical Visualization of Binding of the Chemical Carcinogen Benzo(a)pyrene Diol-Epoxide 1 to the Genome

Laboratory of Molecular Carcinogenesis, National Cancer Institute, NIH, Bethesda, Maryland 20205

Antisera against DNA modified with r-7,t-8-dihydroxy-t-9,10-oxy-7,8,9,10-tetrahydrobenzo(a)pyrene (BPDE-1) was elicited in rabbits. Such sera reacted with either single- or double-stranded modified DNA but not with unmodified DNA, free benzo(a)pyrene, or proteins modified by BPDE-1.

Indirect immunofluorescence studies indicated that the immunoglobulin G in the sera bound specifically to the nuclei of KD cells which were treated with BPDE-1. The intensity of fluorescence was proportional to the dose of BPDE-1 used to treat the cells. About 50% of the BPDE-1-DNA adducts remained bound to DNA 24 hr after the removal of the carcinogen. The location of BPDE-1-modified bases in Col E1 DNA was visualized by immunoelectron microscopy.

¹ Permanent address: Department of Human Genetics, Tel Aviv University, School of Medicine, Tel Aviv, Israel.

² To whom requests for reprints should be addressed, at Building 37, Room 3D20, NIH, Bethesda, Md. 20205.

Received 1/ 7/81. Accepted 5/ 7/81.