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Comparative Effects of Polycythemia-inducing Friend Leukemia Virus and Moloney Leukemia Virus on Hematopoietic Progenitors in Murine Long-Term Bone Marrow Cultures¹

Laboratoire Immunologie et Virologie des Tumeurs, INSERM U 152, CNRS ERA 781 [J. M. H., S. G., B. V.] and Poste de Transfusion [S. F.], Hôpital Cochin, 27 rue du Faubourg Saint-Jacques, 75674 Paris Cédex 14, France

The specificity of murine leukemia virus-induced myelomonocytic phenotypic changes in long-term bone marrow culture have been examined by comparing the effects of polycythemia-inducing Friend leukemia virus (FV_p) and Moloney murine leukemia virus (M-MuLV) known to have in vivo target cells in the erythroid and lymphoid lineage, respectively. Noninfected and M-MuLV-infected cultures showed no modification in granulocyte macrophage colony-forming cell behavior and failed to generate cell line in WEHI-3-conditioned medium. In contrast, in FV_p-infected cultures, granulocyte macrophage colony-forming cells became colony-stimulating factor independent, and the nonadherent cells gave rise to two cell lines in WEHI-3 conditioned medium with monocytic characteristics and no leukemogenic potential in vivo. These results confirm the ability of long-term bone marrow culture to unmask target cells for FV_p within myelomonocytic progenitors, and the negative results in M-MuLV-infected cultures underline the specificity of the FV_p-induced phenotypic changes. Despite a high level of virus production and the presence of T-cell precursors in the M-MuLV infected culture, T-cell transformation was not observed.

¹ This work was supported by INSERM Grant 82.79.114 No. 28 and DGRST Grant 78.7.2636.

² To whom requests for reprints should be addressed, at Laboratoire Immunologie et Virologie des Tumeurs, INSERM U 152, Hôpital Cochin, 27 rue du Faubourg Saint-Jacques, 75674 Paris Cédex 14, France.

Received 12/10/80. Accepted 5/12/81.