This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by McIntyre, L. J. Articles by Kim, Y. S. Search for Related Content PubMed PubMed Citation Articles by McIntyre, L. J. Articles by Kim, Y. S.

Attachment of Human Pancreatic Tumor Cell Lines to Collagen in Vitro¹

Gastrointestinal Research Laboratory, Veterans Administration Medical Center and Department of Medicine, University of California School of Medicine, San Francisco, California 94143 [L. J. M., Y. S. K.], and Laboratory of Developmental Biology and Anomalies, National Institute of Dental Research, NIH, Bethesda, Maryland 20205 [H. K. K., G. R. M.]

The attachment to purified collagens of three cell lines established from human pancreatic carcinomas was investigated. PANC-1 and CAPAN-1 cells attached to type I, III, and IV collagens in the absence of fetal bovine serum. MIA PaCa-2 cells, on the other hand, attached only to type IV collagen. In the case of MIA PaCa-2 cells, the attachment occurs more slowly and to a lesser extent. Increasing concentrations of fetal bovine serum had no effect on the attachment of PANC-1 and CAPAN-1 cells to the collagens. However, the attachment of MIA PaCa-2 cells to all the collagen types was greatly enhanced hanced by 10% fetal bovine serum. This enhancement was shown to be due to the fibronectin present in the serum.

¹ This work was supported by USPHS Grant CA-24321 from the National Cancer Institute through the National Pancreatic Cancer Project and by the Veterans Administration Medical Research Service.

² To whom requests for reprints should be addressed.

Received 2/13/81. Accepted 5/20/81.