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Monoclonal Antibodies to Carcinoembryonic Antigen Produced by Somatic Cell Fusion¹

Mallory Gastrointestinal Research Laboratory, Boston City Hospital, Boston 02118 [H. Z. K., N. Z.]; Infectious Disease [V. R. Z., P. H. B.] and Cardiac Units [V. R. Z., J. G. R. H.], Department of Medicine, Massachusetts General Hospital, Boston 02114; Department of Medicine [V. R. Z., N. Z., P. M. B.], Harvard Medical School, Boston 02114; and Departments of Microbiology [H. Z. K., P. H. B.] and Pathology [N. Z.], Boston University School of Medicine, Boston, Massachusetts 02118

Hybridoma cell lines secreting monoclonal antibodies to carcinoembryonic antigen (CEA) were generated by fusing mouse immune lymphocytes with the mouse myeloma variant cell line, NS-1. Antibody secreted by one cloned hybrid cell line could bind only a select portion of the CEA bound by the commercially available goat anti-CEA antiserum used in clinical assays. Radiolabeled CEA could be purified on a monoclonal antibody affinity column. Incorporation of this purified radiolabeled CEA in a double-antibody solid-phase assay with goat anti-CEA antiserum led to an approximately 2.5-fold increase in sensitivity of the assay. Genetically stable hybrid clones may be sources of virtually unlimited quantities of such antibodies which may have potential utility in improving the cancer specificity of clinical assays.

¹ This research was supported by USPHS Grants CA-04486 and CA-10126 from the National Cancer Institute.

² Present address: Department of Microbiology, Boston University School of Medicine, 80 E. Concord Street, Boston, Mass. 02118.

³ To whom requests for reprints should be addressed.

⁴ Completed in part during tenure as Fellow of the Research Foundation, Boston, Mass. Additional salary support was provided by USPHS Grants CA-10126 to Dr. Black and CA-24432 to Dr. Edgar Haber. Present address: Centocor, 3508 University City Science Center, Philadelphia, Pa. 19104.

⁵ Recipient of a Fulbright Fellowship from the Australian-American Educational Foundation while on study leave from the Commonwealth Serum Laboratories, Parkville, Victoria, Australia. Present address: Research and Development Division, Commonwealth Serum Laboratories, Parkville, Victoria 3052, Australia.

Received 7/18/80. Accepted 5/15/81.