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Effects of Bile Acids on Colon Carcinogenesis in Rats Treated with Carcinogens¹

New York Veterans' Administration Medical Center, 408 First Avenue, New York 10010, and Department of Medicine, New York University Medical Center, 555 First Avenue, New York, New York 10016

Primary bile acids were studied as possible colon tumor promoters or inhibitors in a rat model of chemically induced colon cancer. Cholic acid feeding increased the number of animals with tumors, the number of tumors per animal, and the number of tumors per tumor-bearing animal. Tumor enhancement was attributed to deoxycholic acid, the bacterial metabolite of cholic acid. When chenodeoxycholic acid was fed to the rats in our model, tumor incidence was increased, but the number of tumors per animal and the number of tumors per tumor-bearing animal were similar to controls. The different fecal bile acid pattern obtained with chenodeoxycholic acid may be responsible for the differences in tumor incidence.

The methodology to characterize and identify all steroidal components of the feces requires extraction, thin-layer chromatography, gas-liquid chromatography, and gas-liquid chromatography-mass spectrometry. Newer techniques include LH-20 chromatography (for sulfated steroids) and high-pressure liquid chromatography.

¹ Presented at the Workshop on Fat and Cancer, December 10 to 12, 1979, Bethesda, Md. Supported by Grant 27438 from the National Cancer Institute through the National Large Bowel Cancer Project.

² Recipient of a grant from the Veterans Administration.