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Correlation between Activation of Quiescent 3T3 Cells by Retinoic Acid and Increases in Uridine Phosphorylation and Cellular RNA Synthesis

Department of Microbiology, Boston University School of Medicine, and The Hubert H. Humphrey Cancer Research Center at Boston University, Boston, Massachusetts 02118

Short exposure of cultured quiescent cells to micromolar quantities of ß-all-trans-retinoic acid (RA) has been reported to potentiate the effects of phorbol myristate acetate in promoting the transition from the resting to growing states of these cells. Longer periods of exposure to RA result in substantial inhibition of cellular proliferation. We now show that short-term treatment of quiescent Swiss 3T3 cells with RA yields marked increases in uridine phosphorylation and total cellular RNA synthesis as well as 2-deoxyglucose uptake. Upon subsequent treatment of the cells with phorbol myristate acetate, a direct correlation between the comitogenic activity of RA and its stimulation of uridine phosphorylation and RNA synthesis is apparent. The increases in 2-deoxyglucose uptake persist after long-term exposure of the cells to RA when the growth-inhibitory effects of this agent are observed.

¹ Supported by Research Grant CA-28803. To whom requests for reprints should be addressed.

² Supported by Research Grant IN-97F from the American Cancer Society.

³ Supported by Research Grant CA-28107 from the National Cancer Institute.

Received 6/ 7/82. Accepted 8/13/82.