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Glycosaminoglycan Synthesis by a Cell Line (C1-S1) Established from a Preneoplastic Mouse Mammary Outgrowth¹

Department of Zoology, Washington State University, Pullman, Washington 99164-4220

We have measured the synthesis of several types of glycosaminoglycans by a line of mouse mammary epithelial cells (C1-S1) established from a hyperplastic nodule outgrowth. These epithelioid cells do not grow readily in vivo. Subconfluent monolayer cultures of C1-S1 cells produced more hyaluronic acid than heparan sulfate, but the opposite was true in confluent cultures. At saturation density in culture, the cell surface glycosaminoglycan of C1-S1 cells was approximately 80% heparan sulfate. For comparison, data are also reported on two related tumorigenic sublines (+SA and -SA) established from a spontaneous tumor in a hyperplastic outgrowth. These cells produced mostly hyaluronic acid even when confluent. Furthermore, the net rate of hyaluronic acid synthesis was higher in the more aggressive tumor cells (+SA). The data are consistent with the interpretation that a hyaluronic acid-rich, heparan sulfate-poor environment is associated with the growth of mammary epithelial cells and conversely that a heparan sulfate-rich environment may restrict growth. The glycosaminoglycan environment may thus contribute to growth modulation in vivo.

¹ Supported by NIH Grant CA-16392 and Contract NO1-CB-63986 from the Breast Cancer Task Force of the National Cancer Institute.

² Present address: Department of Biological Structure, SM-20, University of Washington, Seattle, Wash. 98195.

³ To whom requests for reprints should be addressed.

⁴ Recipient of postdoctoral fellowships from NIH (CA-05732) and the American Cancer Society (PF-1473). Present address: Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Wash. 99164.

Received 5/ 3/82. Accepted 9/ 9/82.

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