This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Brothman, A. R. Articles by Lindell, T. J. Search for Related Content PubMed PubMed Citation Articles by Brothman, A. R. Articles by Lindell, T. J.

Development of an Antibody to Actinomycin D and Its Application for the Detection of Serum Levels by Radioimmunoassay¹

Department of Pharmacology, Arizona Health Sciences Center, Tucson, Arizona 85724

An antibody specific for actinomycin D (Act D) has been developed and used in a rapid, sensitive radioimmunoassay for detection of this anticancer drug in serum. The 2-amino group of the heterocyclic chromophore of Act D was covalently coupled to available free carboxyl groups of bovine serum albumin with carbodiimide. The resulting complex was then used for the production of a specific antibody to Act D in two male New Zealand rabbits. Antibody production was of sufficient titer in both rabbits to allow the development of a radioimmunoassay for the free drug which is rapid and sensitive enough to accurately measure 0.1 pmol of Act D. The antibody produced was characterized to be immunoglobulin G by virtue of its ability to bind to Protein A:Sepharose columns. With the use of Act-D analog, actinomine, the antibody was characterized to be specific for the pentapeptide portion of the molecule. Pharmacokinetic analysis of serial serum samples obtained from a patient who received the drug i.v. revealed a biphasic response with an -serum half-life of 1.78 min and a ß serum half-life of 34 min. An i.v. injection of Act D into a dog and assay of serum concentration revealed a similar biphasic response with an serum half-life of 0.78 min and a ß-serum half-life of of 208 min.

¹ This work was supported by Grant GM-22897.

² To whom requests for reprints should be addressed.

Received 9/29/81. Accepted 11/13/81.

This article has been cited by other articles:

				G. J. Veal, M. Cole, J. Errington, A. Parry, J. Hale, A. D.J. Pearson, K. Howe, J. C. Chisholm, C. Beane, B. Brennan, et al. Pharmacokinetics of Dactinomycin in a Pediatric Patient Population: a United Kingdom Children's Cancer Study Group Study Clin. Cancer Res., August 15, 2005; 11(16): 5893 - 5899. [Abstract] [Full Text] [PDF]