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1,25-Dihydroxyvitamin D₃ Receptors in Human Epithelial Cancer Cell Lines¹

Department of Medicine, Stanford University School of Medicine, Stanford, California 94305 [K. C., M. J. C., A. H. F., D. F.], and Division of Life Sciences, SRI International, Menlo Park, California 94025 [M. J. C., A. H. F.]

Specific, high-affinity cytosolic receptors for 1,25-dihydroxyvitamin D₃ have been demonstrated in five human cancer cell lines. The cell lines were derived from tumors of breast, lung, cervix, and melanotic and amelanotic melanomas. Binding affinity (K_d) of the receptors for 1,25-dihydroxyvitamin D₃ were all approximately 0.2 nM, and receptor content ranged from 21 to 174 fmol/mg cytosol protein. The receptors from all five cell lines sedimented at 3.2S on sucrose density gradients and exhibited preferential affinity for 1,25-dihydroxyvitamin D₃ compared to other vitamin D metabolites.

¹ Supported by NIH Grant AG 01312.

² To whom requests for reprints should be addressed, at Division of Endocrinology, S-005, Stanford University School of Medicine, Stanford, Calif. 94305.

Received 8/ 7/81. Accepted 11/18/81.

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